Title : Eugenol-derived Self-Assembled Nanoparticles (E-SAN) as a functional feed in white shrimp Litopenaeus vannamei
Abstract:
Eugenol-derived self-assembled nanoparticles (E-SAN) offer potential benefits to shrimp immunity due to their antioxidant and antimicrobial properties. This study evaluates the effects of E-SAN on growth performance and shrimp immunity, particularly its protection against Vibrio parahaemolyticus infection (AHPND). The experiment was divided into two groups: one group was subjected to challenge tests, while the other was used to observe growth performance. Twenty shrimp, with an average body weight of 4.6 ± 0.3 g per shrimp, were reared in aquariums (60 × 45 × 40 cm³) containing 96 L of filtered seawater in a circulatory system maintained at a temperature of 28°C. The shrimp were fed commercial pellets supplemented with three different concentrations of E-SAN: 0 μg g-1, 200 μg g-1, and 400 μg g-1. The results revealed that functional feeds containing E-SAN protected the shrimp from AHPND without negatively affecting their growth performance. The protective effects of E-SAN were observed as early as the first week of administration and persisted until the end of the culture period. However, further analysis is needed to optimize the dosage, as histological examination of the hepatopancreas showed structural changes. These findings suggest that E-SAN has great potential as a dietary supplement to enhance shrimp health and resilience against infectious diseases.
