Title : First report of Anthocyanin as nutralizer against Takumi (Flubendiamide 20% WDG) induced toxicity in Labeo rohita
Abstract:
Takumi (Flubendiamide 20% WDG) insecticide is used against various borer insect pests like, stem borer, pod borer, leaf folder, semilooper etc. Borer insects are major insect pests of Rice crop. Hence, the insecticide is popularly used to manage the borer pests in paddy fields. These agrochemicals after absorption, leaching and adsorption enters into the aquatic body producing severe toxication in non-target organisms. The commonly occurring fingerlings of Labeo rohita is exposed to Takumi in rice fields. Long term chronic toxicity (30, 60 and 90 days) was studied in Labeo rohita against the insecticide Takumi (Flubendiamide 20% WDG). The present investigation involves changes expressed at biochemical, haematological, histological, protein profile and genotoxic level. Significant decrease was observed for different biochemical parameters like, total protein, serum albumin and A/G ratio whereas some parameters increased, like glucose, cholesterol, SGOT, SGPT, creatinine, alkaline phosphatase and cortisol. Among Haematological parameters, significant decrease was observed for hemoglobin, RBC, PCV, MCHC and neutrophil. In contrary, WBC, monocyte and eosinophil showed increase in concentration. The degradation of DNA was highest in liver (-88.26) followed by muscle (-87.26) and heart (-79.67). Anthocyanins, glycosides of anthocyanidins, are phenolic compoundscausing purple colour in different crops. It has antidiabetic, anticancer, anti-inflammatory, antimicrobial, and anti-obesity effects, as well as used as preventive approach against cardiovascular diseases. Anthocyanins extracted from edible parts of plants have potential to treat toxicityin aquatic organisms.For curative measure, anthocyanin was isolated from purple fleshed sweet potato, DOP-92-120, without using alcohol or acetone and injected into the caudal vein of Labeo rohita. Administration of anthocyanin at a dose of 5mg/ml caused significant remedial feature in the non-target organism.
What will audience learn from your presentation?
- Levels of toxicity induced due to excessive application of insecticide (Flubendiamide 20% WDG) in non-target organisms
- How different Biochemical, haematological, histological, protein profile and genotoxic parameters vary under insecticide intoxication
- How Anthocyanin may be isolated without using alcohol and acetone
- Curative measures of Anthocyanin against insecticide toxicity
