Title : Effect of preservation method and disinfectants on the hatching of freshwater rotifer resting eggs Brachionus calyciflorus
Abstract:
With the development of larval-rearing technology for freshwater fishes, the demand for rotifers is increasing. The use of resting eggs is also highly recommended to prevent pathogen contamination from live feed routes, as well as to significantly reduce labor costs and algae production costs for scaling up farming. Two experiments were carried out to determine the quantitative effect of preservation methods and disinfectants on the hatching rate of freshwater rotifer resting egg Brachionus calyciflorus. The first experiment examined the effect of storage methods on the hatching rates of resting eggs. After 120 days of storage, resting eggs preserved by dry, cold and dark methods have hatching rates ranging from 6.76% to 22.15%; resting eggs held by wet, cold, and dark methods had higher hatching rates from 25.28% to 46.4%. The second experiment examined the effect of preservatives on the hatching rate of resting eggs in combination with wet, cold and dark methods. For the antibiotic Doxycycline (20μg/ml), the hatching rate was 31.87 ± 5.27%. When disinfecting with Anolyte (10%; 20%; 30%; 50%; 100%), the hatching rate of rotifer resting eggs was 12.38%; 17.95%; 4.71%; 2.36%; 0%, respectively. At the concentrations of Nanosilver 1, 2, 3, and 4ppm, the hatching rate was 30.5%, 23.6%, 27.47% and 17.64%, respectively. In conclusion, preserving freshwater rotifer eggs Brachionus calyciflorus is done by wet, cold, and dark methods. It can use disinfectants Doxycycline (20μg/ml) or Nanosilver with a concentration of 1 ppm to 3 ppm to disinfect resting eggs before incubation effectively increasing the hatching rate of resting eggs.
What will the audience learn from your presentation?
- This study provides some additional information on preservation methods and the selection of suitable disinfectants to increase the hatching rate of freshwater rotifer resting eggs Brachionus calyciflorus.
- The present research of this study can assist in cultivating freshwater rotifers for application in the hatchery of freshwater aquatic species.
- In Vietnam, there are not many studies on freshwater rotifers Brachionus calyciflorus; this is also a research direction that can be exploited in the future.
