Title : Isolation and determination of Vibrio spp. pathogen from Sciaenops ocellatus suffering from hemorrhagic disease in in under cage culture in Vietnam
Abstract:
This study was carried out to isolate and determine the Vibrio spp. from the Red drum fish (Sciaenops ocellatus) suffering from hemorrhagic disease in Vietnam. In this study, 18 strains of Vibrio bacteria were identified from 27 samples of Red drum fish. The isolate bacterial strains were identified with the 16S rRNA sequencing method and checked for morphological, physiological, and biochemical characteristics by using the API 20E KIT. Result of the study revealed the presence of twelve strains of V. alginolyticus, three strains of V. fluvialis, and three strains of V. orientalis. All Vibrio strains have gene similarities with those on the genbank ranging from 98.05 to 100%. The biochemical characteristics of these 18 isolates were similar and these are susceptible to tetracycline and doxycycline and entirely resistant to ampicillin, amoxicillin, and erythromycin.
What will audience learn from your presentation?
- The audience will know the method of isolating and identifying strains of Vibrio spp. causing hemorrhagic disease in Red drum (Sciaenops ocellatus) using PCR and biochemical characteristics by using the API 20E KIT.
- The results of the study are of reference value, a premise for further studies related to Vibrio disease on marine fish in general and Red drum in particular.
